Sequence of the rat alpha c large chain of the clathrin associated protein complex AP-2.

We have isolated cDNA clones for the large etc chain of the rat brain Clathrin Associated Protein complex AP-2. A rat brain XZAP-I1 cDNA library (Stratagene) was screened with the synthetic oligonucleotide probe 5'-C(C, T) TCCAT (A ,G) TGNCC (A, G) AA (A, G) TC (G, T) ATGTCGTG, corresponding to the stretch of amino acids between His-70 and AIa-79 that are shared by both mouse aa and ac chains (1). Two cDNA clones were sequenced by the dideoxy termination method (2) using internal 17-mer sequencing primers. The combined DNA sequence is shown in Fig. 1. Sequence comparison with the mouse aa and «c homologues demonstrates that the rat protein IS 99% identical to the mouse ac chain. It contains 938 amino acids, with no gaps, and two conservative changes corresponding to Glu-8 instead of Asp-8 and De-788 instead of Val-788, respectively.